猪铁调素(Hepc)酶联免疫吸附检测试剂盒

英文名称	Pig Hepc(Hepcidin) ELISA Kit
别名	HAMP; HFE2B; PLTR; LEAP1; Hepcidin Antimicrobial Peptide; Liver-expressed antimicrobial peptide 1; Putative liver tumor regressor

种属	Pig
检测类型	Sandwich
灵敏度	0.222 ng/mL
检测范围	0.63-40 ng/mL
样本类型	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长	3.5h

研究领域	Metabolic pathway;Endocrinology;Hematology;Genetic science;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig Hepc. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig Hepc. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig Hepc, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig Hepc in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	Q8MJ80

标准曲线图

【仅供科学研究使用】

相关产品: