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牛白介素2受体a链(IL-2Ra/CD25)酶联免疫吸附检测试剂盒

  • 货号:ELK7953
  • 价格:¥2,800.00 元
  • 点击:1859
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产品介绍

产品信息

英文名称Cattle IL-2sRa/CD25(Soluble Interleukin-2 Receptor alpha chain) ELISA Kit
别名IL-2RA;CD25; IL2R-a; IL2-RA; IDDM10; IL2R; TCGFR; p55; TAC antigen; IL2Ra; Interleukin 2 Receptor Alpha

规格与价格

48T 价格1960
96T 价格2800
96T*5 价格11900

检测参数

种属Cattle
检测类型Sandwich
灵敏度6.6 pg/mL
检测范围15.63-1000 pg/mL
样本类型Serum, plasma and other biological fluids.
实验时长3.5h

研究信息

研究领域Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle IL-2sRa/CD25. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle IL-2sRa/CD25. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle IL-2sRa/CD25, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle IL-2sRa/CD25 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

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【仅供科学研究使用】

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