小鼠70kDa热休克蛋白1样蛋白(HSPA1L)酶联免疫吸附检测试剂盒

英文名称	Mouse HSPA1L(Heat Shock 70kDa Protein 1 Like Protein) ELISA Kit
别名	HSP-A1L; HSPA1-L; HSP70-HOM; Hum70t

种属	Mouse
检测类型	Sandwich
灵敏度	0.59 ng/mL
检测范围	1.57-100 ng/mL
样本类型	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长	3.5h

研究领域	Signal transduction;Infection immunity;Developmental science;Pulmonology;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse HSPA1L. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse HSPA1L. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse HSPA1L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse HSPA1L in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	P16627

标准曲线图

【仅供科学研究使用】

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