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大鼠膜铁转运辅助蛋白(HEPH)酶联免疫吸附检测试剂盒

  • 货号:ELK7588
  • 价格:¥2,800.00 元
  • 点击:782

产品介绍

产品信息

英文名称Rat HEPH(Hephaestin) ELISA Kit
别名CPL

规格与价格

48T 价格1960
96T 价格2800
96T*5 价格11900

检测参数

种属Rat
检测类型Sandwich
灵敏度0.125 ng/mL
检测范围0.32-20 ng/mL
样本类型Serum, plasma, tissue homogenates and other biological fluids
实验时长3.5h

研究信息

研究领域Hematology;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HEPH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HEPH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HEPH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HEPH in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号Q920H8

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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