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人内向整流型钾离子通道亚家族J成员10(KCNJ10)微量上样酶联免疫吸附检测试剂盒

  • 货号:ELK7412MS
  • 价格:¥2,800.00 元
  • 点击:1984

产品介绍

产品信息

英文名称Human KCNJ10(Potassium Inwardly Rectifying Channel Subfamily J, Member 10) Microsample ELISA Kit
别名Kir4.1; Kir1.2; ATP-sensitive inward rectifier potassium channel 10; ATP-dependent inwardly rectifying potassium channel Kir4.1; Inward rectifier K(+) channel Kir1.2

规格与价格

48T 价格1960
96T 价格2800
96T*5 价格11900

检测参数

种属Human
检测类型Sandwich
灵敏度0.6 ng/mL
检测范围1.57-100 ng/mL
样本类型Tissue homogenates, cell lysates and other biological fluids
实验时长3.5h

研究信息

研究领域Signal transduction;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KCNJ10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KCNJ10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KCNJ10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KCNJ10 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号P78508

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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