大鼠驱动蛋白家族成员5B(KIF5B)酶联免疫吸附检测试剂盒

英文名称	Rat KIF5B(Kinesin Family, Member 5B) ELISA Kit
别名	KNS1; UKHC; KINH; Conventional kinesin heavy chain; Ubiquitous kinesin heavy chain; Kinesin-1 heavy chain

种属	Rat
检测类型	Sandwich
灵敏度	0.058 ng/mL
检测范围	0.16-10 ng/mL
样本类型	Tissue homogenates and other biological fluids.
实验时长	3.5h

研究领域	Signal transduction;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat KIF5B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat KIF5B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat KIF5B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat KIF5B in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	Q2PQA9

标准曲线图

【仅供科学研究使用】

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