小鼠溶酶体酸脂肪酶A(LIPA)酶联免疫吸附检测试剂盒

英文名称	Mouse LIPA(Lipase A, Lysosomal Acid) ELISA Kit
别名	LAL; CESD; Lipase A,Lysosomal Acid,Cholesterol Esterase(Wolman Disease); Acid Cholesteryl Ester Hydrolase; Lysosomal acid lipase/cholesteryl ester hydrolase

种属	Mouse
检测类型	Sandwich
灵敏度	0.143 ng/mL
检测范围	0.32-20 ng/mL
样本类型	serum, plasma, tissue homogenates and other biological fluids
实验时长	3.5h

研究领域	Enzyme & Kinase;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse LIPA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse LIPA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse LIPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse LIPA in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	Q9Z0M5

标准曲线图

【仅供科学研究使用】

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