大鼠非转移细胞1表达NM23A蛋白(NME1)酶联免疫吸附检测试剂盒
- 货号：ELK6813
- 价格：￥2,800.00 元
- 点击：670
产品介绍
产品信息
| 英文名称 | Rat NME1(Non Metastatic Cells 1, Protein NM23A Expressed In) ELISA Kit |
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| 别名 | AWD; GAAD; NDPKA; NM23-H1; Nucleoside diphosphate kinase A; Granzyme A-activated DNase; Metastasis inhibition factor nm23; Tumor metastatic process-associated protein |
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规格与价格
| 48T 价格 | 1960 |
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| 96T 价格 | 2800 |
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| 96T*5 价格 | 11900 |
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检测参数
| 种属 | Rat |
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| 检测类型 | Sandwich |
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| 灵敏度 | 0.113 ng/mL |
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| 检测范围 | 0.32-20 ng/mL |
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| 样本类型 | Serum, plasma and other biological fluids. |
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| 实验时长 | 3.5h |
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研究信息
| 研究领域 | Signal transduction; |
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| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NME1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NME1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NME1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NME1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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标准曲线
| 标准曲线图 |  |
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