人角蛋白33A(KRT33A)微量上样酶联免疫吸附检测试剂盒

英文名称	Human KRT33A(Keratin 33A) Microsample ELISA Kit
别名	KRTHA3A; Ha-3I; Krt1-3; K33A; HHA3-I; HKA3A; Keratin,Hair,Acidic 3A; Hard Keratin Type I 3I; Keratin, type I cuticular Ha3-I

种属	Human
检测类型	Sandwich
灵敏度	29 pg/mL
检测范围	78.13-5000 pg/mL
样本类型	Serum, plasma and other biological fluids.
实验时长	3.5h

研究领域	Tumor immunity;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KRT33A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KRT33A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KRT33A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KRT33A in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	O76009

标准曲线图

【仅供科学研究使用】

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