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大鼠生长分化因子5(GDF5)微量上样酶联免疫吸附检测试剂盒

  • 货号:ELK6711MS
  • 价格:¥2,800.00 元
  • 点击:1978
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产品介绍

产品信息

英文名称Rat GDF5(Growth Differentiation Factor 5) Microsample ELISA Kit
别名CDMP1; LAP4; SYNS2; BMP14; Radotermin; Cartilage-Derived Morphogenetic Protein-1; Bone morphogenetic protein 14; Lipopolysaccharide-associated protein 4

规格与价格

48T 价格1960
96T 价格2800
96T*5 价格11900

检测参数

种属Rat
检测类型Sandwich
灵敏度0.06 ng/mL
检测范围0.16-10 ng/mL
样本类型Serum, plasma, tissue homogenates and other biological fluids.
实验时长3.5h

研究信息

研究领域Cytokine;Signal transduction;Neuro science;Developmental science;Bone metabolism;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat GDF5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GDF5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat GDF5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GDF5 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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