人信号素4A(SEMA4A)酶联免疫吸附检测试剂盒

英文名称	Human SEMA4A(Semaphorin 4A) ELISA Kit
别名	CORD10; RP35; SEMAB; SEMB; Semaphorin-B; Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 4A

种属	Human
检测类型	Sandwich
灵敏度	12.8 pg/mL
检测范围	31.25-2000 pg/mL
样本类型	serum, plasma, tissue homogenates, cell lysates and other biological fluids
实验时长	3.5h

研究领域	CD & Adhesion molecule;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SEMA4A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SEMA4A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SEMA4A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SEMA4A in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	Q9H3S1

标准曲线图

【仅供科学研究使用】

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