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小鼠氢/钾离子交换ATP酶α肽(ATP4a)酶联免疫吸附检测试剂盒

  • 货号:ELK6337
  • 价格:¥2,800.00 元
  • 点击:1514

产品介绍

产品信息

英文名称Mouse ATP4a(ATPase, H+/K+ Exchanging Alpha Polypeptide) ELISA Kit
别名Gastric H,K-ATPase Alpha Subunit; H(+)-K(+)-ATPase Alpha Subunit; Proton Pump; Potassium-transporting ATPase alpha chain 1

规格与价格

48T 价格1960
96T 价格2800
96T*5 价格11900

检测参数

种属Mouse
检测类型Sandwich
灵敏度0.053 ng/mL
检测范围0.16-10 ng/mL
样本类型Tissue homogenates and other biological fluids.
实验时长3.5h

研究信息

研究领域Signal transduction;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ATP4a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ATP4a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ATP4a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ATP4a in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号Q64436

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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