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人信号素3F(SEMA3F)酶联免疫吸附检测试剂盒

  • 货号:ELK6313
  • 价格:¥2,800.00 元
  • 点击:1690

产品介绍

产品信息

英文名称Human SEMA3F(Semaphorin 3F) ELISA Kit
别名SEMA-IV; SEMA4; SEMAK; Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 3F

规格与价格

48T 价格1960
96T 价格2800
96T*5 价格11900

检测参数

种属Human
检测类型Sandwich
灵敏度5.9 pg/mL
检测范围15.63-1000 pg/mL
样本类型serum, plasma, tissue homogenates and other biological fluids
实验时长3.5h

研究信息

研究领域CD & Adhesion molecule;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SEMA3F. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SEMA3F. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SEMA3F, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SEMA3F in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号Q13275

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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