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大鼠中性鞘磷脂酶(NSMASE)微量上样酶联免疫吸附检测试剂盒

  • 货号:ELK6191MS
  • 价格:¥2,800.00 元
  • 点击:1381

产品介绍

产品信息

英文名称Rat NSMASE(Neutral Sphingomyelinase) Microsample ELISA Kit
别名SMPD2; N-SMase; ISC1; Neutral Sphingomyelin Phosphodiesterase; Sphingomyelin Phosphodiesterase 2,Neutral Membrane; Lyso-platelet-activating factor-phospholipase C

规格与价格

48T 价格1960
96T 价格2800
96T*5 价格11900

检测参数

种属Rat
检测类型Sandwich
灵敏度0.064 ng/mL
检测范围0.16-10 ng/mL
样本类型Tissue homogenates and other biological fluids.
实验时长3.5h

研究信息

研究领域Enzyme & Kinase;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NSMASE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NSMASE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NSMASE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NSMASE in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号Q9ET64

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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