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人ⅣD组磷脂酶A2(PLA2G4D)微量上样酶联免疫吸附检测试剂盒

  • 货号:ELK6188MS
  • 价格:¥2,800.00 元
  • 点击:1775

产品介绍

产品信息

英文名称Human PLA2G4D(Phospholipase A2, Group IVD) Microsample ELISA Kit
别名cPLA2delta; Cytosolic phospholipase A2 delta

规格与价格

48T 价格1960
96T 价格2800
96T*5 价格11900

检测参数

种属Human
检测类型Sandwich
灵敏度0.063 ng/mL
检测范围0.16-10 ng/mL
样本类型Tissue homogenates and other biological fluids.
实验时长3.5h

研究信息

研究领域Enzyme & Kinase;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PLA2G4D. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PLA2G4D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PLA2G4D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PLA2G4D in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号Q86XP0

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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