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大鼠皮质醇结合球蛋白(CBG)微量上样酶联免疫吸附检测试剂盒

  • 货号:ELK6114MS
  • 价格:¥2,800.00 元
  • 点击:1518

产品介绍

产品信息

英文名称Rat CBG(Corticosteroid Binding Globulin) Microsample ELISA Kit
别名SERPINA6; Transcortin; Serpin A6; Serpin Peptidase Inhibitor,Clade A,Member 6(Alpha-1 Antiproteinase; Antitrypsin)

规格与价格

48T 价格1960
96T 价格2800
96T*5 价格11900

检测参数

种属Rat
检测类型Sandwich
灵敏度6.7 ng/mL
检测范围15.63-1000 ng/mL
样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长3.5h

研究信息

研究领域Endocrinology;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CBG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CBG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CBG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CBG in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号P31211

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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