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大鼠肿瘤坏死因子配体超家族成员12(TNFSF12)酶联免疫吸附检测试剂盒

  • 货号:ELK6029
  • 价格:¥2,800.00 元
  • 点击:1950

产品介绍

产品信息

英文名称Rat TNFSF12(Tumor Necrosis Factor Ligand Superfamily, Member 12) ELISA Kit
别名APO3L; DR3LG; TWEAK; APO3 ligand; TNF-related weak inducer of apoptosis

规格与价格

48T 价格1960
96T 价格2800
96T*5 价格11900

检测参数

种属Rat
检测类型Sandwich
灵敏度13.6 pg/mL
检测范围31.25-2000 pg/mL
样本类型Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
实验时长3.5h

研究信息

研究领域Tumor immunity;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TNFSF12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TNFSF12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TNFSF12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TNFSF12 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号Q6AYC1

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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