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山羊基质金属蛋白酶13(MMP13)酶联免疫吸附检测试剂盒

  • 货号:ELK5942
  • 价格:¥2,800.00 元
  • 点击:1791
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产品介绍

产品信息

英文名称Goat MMP13(Matrix Metalloproteinase 13) ELISA Kit
别名CLG3; Collagenase 3

规格与价格

48T 价格1960
96T 价格2800
96T*5 价格11900

检测参数

种属Goat
检测类型Sandwich
灵敏度1.22 ng/mL
检测范围3.13-200 ng/mL
样本类型Serum, plasma and other biological fluids.
实验时长3.5h

研究信息

研究领域Enzyme & Kinase;Tumor immunity;Bone metabolism;Rheumatology;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Goat MMP13. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Goat MMP13. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Goat MMP13, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Goat MMP13 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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