马白介素1受体拮抗剂(IL1RA)酶联免疫吸附检测试剂盒

英文名称	Horse IL1RA(Interleukin 1 Receptor Antagonist) ELISA Kit
别名	IL1RN; IL1-RA; ICIL1RA; IL1ra3; IL1F3; IRAP; IL-1RA; Interleukin-1 Family Member 3; IL1 inhibitor; Anakinra

种属	Horse
检测类型	Sandwich
灵敏度	28 pg/mL
检测范围	78.13-5000 pg/mL
样本类型	Serum, plasma and other biological fluids.
实验时长	3.5h

研究领域	Cytokine;Signal transduction;Infection immunity;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse IL1RA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse IL1RA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse IL1RA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse IL1RA in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	O18999

标准曲线图

【仅供科学研究使用】

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