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牛白介素12A(IL12A)酶联免疫吸附检测试剂盒

  • 货号:ELK5873
  • 价格:¥2,800.00 元
  • 点击:1976

产品介绍

产品信息

英文名称Cattle IL12A(Interleukin 12A) ELISA Kit
别名IL-12A;p35; IL12-A; CLMF1; NFSK; NKSF1; Natural Killer Cell Stimulatory Factor 1; Cytotoxic Lymphocyte Maturation Factor 1; NF Cell Stimulatory Factor Chain 1

规格与价格

48T 价格1960
96T 价格2800
96T*5 价格11900

检测参数

种属Cattle
检测类型Sandwich
灵敏度3.2 pg/mL
检测范围7.82-500 pg/mL
样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长3.5h

研究信息

研究领域Cytokine;Infection immunity;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle IL12A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle IL12A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle IL12A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle IL12A in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号P54349

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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