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豚鼠调节激活正常T-细胞表达分泌因子(RANTES)酶联免疫吸附检测试剂盒

  • 货号:ELK5820
  • 价格:¥2,800.00 元
  • 点击:2686
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产品介绍

产品信息

英文名称Guinea pig RANTES(Regulated On Activation In Normal T-Cell Expressed And Secreted) ELISA Kit
别名CCL5; SCYA5; SISd; EoCP; Chemokine C-C-Motif Ligand 5; Eosinophil Chemotactic Cytokine; T-cell Specific Protein p288; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 5

规格与价格

48T 价格1960
96T 价格2800
96T*5 价格11900

检测参数

种属Guinea pig
检测类型Sandwich
灵敏度0.057 ng/mL
检测范围0.16-10 ng/mL
样本类型Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
实验时长3.5h

研究信息

研究领域Cytokine;Tumor immunity;Infection immunity;Immune molecule;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Guinea pig RANTES. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Guinea pig RANTES. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Guinea pig RANTES, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Guinea pig RANTES in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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