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大鼠Bcl2关联X蛋白(Bax)酶联免疫吸附检测试剂盒

  • 货号:ELK5698
  • 价格:¥2,800.00 元
  • 点击:2247
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产品介绍

产品信息

英文名称Rat Bax(Bcl2 Associated X Protein) ELISA Kit
别名BCL2L4; Bax Zeta; Apoptosis regulator BAX; Bcl-2-like protein 4

规格与价格

48T 价格1960
96T 价格2800
96T*5 价格11900

检测参数

种属Rat
检测类型Sandwich
灵敏度4.7 pg/mL
检测范围12.5-800 pg/mL
样本类型Serum, plasma, tissue homogenates and other biological fluids
实验时长3.5h

研究信息

研究领域Signal transduction;Apoptosis;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Bax. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Bax. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Bax, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Bax in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号Q63690

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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