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人细胞色素P450家族成员21B(CYP21B)酶联免疫吸附检测试剂盒

  • 货号:ELK5576
  • 价格:¥2,600.00 元
  • 点击:964

产品介绍

产品信息

英文名称Human CYP21B(Cytochrome P450 21B) ELISA Kit
别名CYP21A2; CPS1; CA21H; CAH1; CYP21; CYP21-B; 21-OHase; Steroid 21-hydroxylase; Cytochrome P450,Family 21,Subfamily A,Polypeptide 2

规格与价格

48T 价格1820
96T 价格2600
96T*5 价格11050

检测参数

种属Human
检测类型Sandwich
灵敏度0.054 ng/mL
检测范围0.16-10 ng/mL
样本类型tissue homogenates, cell lysates and other biological fluids
实验时长3.5h

研究信息

研究领域Enzyme & Kinase;Metabolic pathway;Endocrinology;Hormone metabolism;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CYP21B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CYP21B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CYP21B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CYP21B in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号P08686

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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