大鼠安眠蛋白1α(MEP1a)酶联免疫吸附检测试剂盒

英文名称	Rat MEP1a(Meprin A Alpha) ELISA Kit
别名	PPHA; PABA Peptide Hydrolase; Endopeptidase-2; N-benzoyl-L-tyrosyl-P-amino-Benzoic Acid Hydrolase Subunit Alpha; PABA Peptide Hydrolase

种属	Rat
检测类型	Competitive Inhibition
灵敏度	15.4 pg/mL
检测范围	62.5-4000 pg/mL
样本类型	serum, plasma, cell lysates, cell culture supernates and other biological fluids
实验时长	2h

研究领域	Metabolic pathway;
检测原理	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat MEP1a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MEP1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MEP1a in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	Q64230

标准曲线图

【仅供科学研究使用】

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