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大鼠弹性蛋白微原纤维界面因子1(EMILIN1)微量上样酶联免疫吸附检测试剂盒

  • 货号:ELK5513MS
  • 价格:¥2,600.00 元
  • 点击:2409

产品介绍

产品信息

英文名称Rat EMILIN1(Elastin Microfibril Interface Located Protein 1) Microsample ELISA Kit
别名EMI; gp115; Elastin Microfibril Interfacer 1

规格与价格

48T 价格1820
96T 价格2600
96T*5 价格11050

检测参数

种属Rat
检测类型Sandwich
灵敏度0.131 ng/mL
检测范围0.32-20 ng/mL
样本类型tissue homogenates, cell lysates, cell culture supernates and other biological fluid
实验时长3.5h

研究信息

研究领域Metabolic pathway;Cardiovascular biology;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat EMILIN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat EMILIN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat EMILIN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat EMILIN1 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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