小鼠摄食抑制因子1(NES1)酶联免疫吸附检测试剂盒
- 货号：ELK5437
- 价格：￥2,600.00 元
- 点击：997
产品介绍
产品信息
| 英文名称 | Mouse NES1(Nesfatin 1) ELISA Kit |
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| 别名 | Nesfatin-1 |
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规格与价格
| 48T 价格 | 1820 |
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| 96T 价格 | 2600 |
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| 96T*5 价格 | 11050 |
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检测参数
| 种属 | Mouse |
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| 检测类型 | Competitive Inhibition |
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| 灵敏度 | 193.2 pg/mL |
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| 检测范围 | 625-40000 pg/mL |
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| 样本类型 | serum, plasma, cell lysates, cell culture supernates and other biological fluids |
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| 实验时长 | 2h |
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研究信息
| 研究领域 | Glycolipid metabolism |
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| 检测原理 | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse NES1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NES1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NES1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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标准曲线
| 标准曲线图 |  |
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