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小鼠摄食抑制因子1(NES1)酶联免疫吸附检测试剂盒

  • 货号:ELK5437
  • 价格:¥2,600.00 元
  • 点击:754

产品介绍

产品信息

英文名称Mouse NES1(Nesfatin 1) ELISA Kit
别名Nesfatin-1

规格与价格

48T 价格1820
96T 价格2600
96T*5 价格11050

检测参数

种属Mouse
检测类型Competitive Inhibition
灵敏度193.2 pg/mL
检测范围625-40000 pg/mL
样本类型serum, plasma, cell lysates, cell culture supernates and other biological fluids
实验时长2h

研究信息

研究领域Glycolipid metabolism
检测原理This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse NES1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NES1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NES1 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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