人NK3同源框蛋白1(NKX3-1)酶联免疫吸附检测试剂盒

英文名称	Human NKX3-1(NK3 Homeobox Protein 1) ELISA Kit
别名	BAPX2; NKX3; NKX3.1; NKX3A; NK3 transcription factor related, locus 1; Homeobox protein NK-3 homolog A

种属	Human
检测类型	Sandwich
灵敏度	0.054 ng/mL
检测范围	0.16-10 ng/mL
样本类型	tissue homogenates, cell lysates and other biological fluids
实验时长	3.5h

研究领域	Signal transduction;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NKX3-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NKX3-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NKX3-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NKX3-1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	Q99801

标准曲线图

【仅供科学研究使用】

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