人20S-蛋白酶体(20S-PSM)酶联免疫吸附检测试剂盒
- 货号：ELK5359
- 价格：￥2,600.00 元
- 点击：974
产品介绍
产品信息
| 英文名称 | Human 20S-PSM(20S-Proteasome) ELISA Kit |
|---|
| 别名 | CP; 20SPSM; 20S Core Particle |
|---|
规格与价格
| 48T 价格 | 1820 |
|---|
| 96T 价格 | 2600 |
|---|
| 96T*5 价格 | 11050 |
|---|
检测参数
| 种属 | Human |
|---|
| 检测类型 | Sandwich |
|---|
| 灵敏度 | 0.66 ng/mL |
|---|
| 检测范围 | 1.57-100 ng/mL |
|---|
| 样本类型 | Tissue homogenates, cell lysates and other biological fluids |
|---|
| 实验时长 | 3.5h |
|---|
研究信息
| 研究领域 | Enzyme & Kinase; |
|---|
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human 20S-PSM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 20S-PSM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human 20S-PSM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 20S-PSM in the samples is then determined by comparing the OD of the samples to the standard curve. |
|---|
标准曲线
| 标准曲线图 |  |
|---|
