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人角蛋白15(KRT15)微量上样酶联免疫吸附检测试剂盒

  • 货号:ELK5185MS
  • 价格:¥2,600.00 元
  • 点击:1990
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产品介绍

产品信息

英文名称Human KRT15(Keratin 15) Microsample ELISA Kit
别名CK15; K15, CK15, K1CO; KRTB; Cytokeratin 15; Keratin-15,Basic; Keratin-15,Beta; Keratin, type I cytoskeletal 15

规格与价格

48T 价格1820
96T 价格2600
96T*5 价格11050

检测参数

种属Human
检测类型Sandwich
灵敏度13.6 pg/mL
检测范围31.25-2000 pg/mL
样本类型Serum, plasma, tissue homogenates and other biological fluids
实验时长3.5h

研究信息

研究领域Tumor immunity;Infection immunity;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KRT15. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KRT15. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KRT15, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KRT15 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号P19012

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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