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大鼠磷脂酰肌醇蛋白聚糖4(GPC4)酶联免疫吸附检测试剂盒

  • 货号:ELK5161
  • 价格:¥2,600.00 元
  • 点击:1308

产品介绍

产品信息

英文名称Rat GPC4(Glypican 4) ELISA Kit
别名K-Glypican; Glypican Proteoglycan 4; Secreted glypican-4

规格与价格

48T 价格1820
96T 价格2600
96T*5 价格11050

检测参数

种属Rat
检测类型Sandwich
灵敏度6.3 pg/mL
检测范围15.63-1000 pg/mL
样本类型Serum, plasma, tissue homogenates and other biological fluids.
实验时长3.5h

研究信息

研究领域Tumor immunity;Developmental science;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat GPC4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GPC4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat GPC4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GPC4 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号Q642B0

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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