人氨基乙二酸半醛磷酸泛酰巯基乙胺基转移酶(AASDHPPT)酶联免疫吸附检测试剂盒

英文名称	Human AASDHPPT(Aminoadipate Semialdehyde Phosphopantetheinyl Transferase) ELISA Kit
别名	AASD-PPT; CGI80; LYS2; LYS5; 4'-phosphopantetheinyl transferase; LYS5 ortholog; Alpha-aminoadipic semialdehyde dehydrogenase-phosphopantetheinyl transferase

种属	Human
检测类型	Sandwich
灵敏度	0.054 ng/mL
检测范围	0.16-10 ng/mL
样本类型	Tissue homogenates and other biological fluids.
实验时长	3.5h

研究领域	Enzyme & Kinase;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AASDHPPT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AASDHPPT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human AASDHPPT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AASDHPPT in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	Q9NRN7

标准曲线图

【仅供科学研究使用】

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