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小鼠蛋白激酶Cα相互作用蛋白1(PICK1)酶联免疫吸附检测试剂盒

  • 货号:ELK4577
  • 价格:¥2,600.00 元
  • 点击:1699

产品介绍

产品信息

英文名称Mouse PICK1(Protein Interacting With Protein Kinase C Alpha 1) ELISA Kit
别名PRKCABP; Protein kinase C,Alpha Binding Protein; Protein interacting with C kinase 1; Protein kinase C-alpha-binding protein

规格与价格

48T 价格1820
96T 价格2600
96T*5 价格11050

检测参数

种属Mouse
检测类型Sandwich
灵敏度0.053 ng/mL
检测范围0.16-10 ng/mL
样本类型Tissue homogenates, cell lysates and other biological fluids.
实验时长3.5h

研究信息

研究领域Signal transduction;Enzyme & Kinase;Metabolic pathway;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PICK1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PICK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PICK1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PICK1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号Q62083

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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