人B-Raf原癌基因丝氨酸/苏氨酸蛋白激酶(BRAF)酶联免疫吸附检测试剂盒
- 货号：ELK4445
- 价格：￥2,600.00 元
- 点击：1503
产品介绍
产品信息
| 英文名称 | Human BRAF(B-Raf Proto Oncogene Serine/Threonine Protein Kinase) ELISA Kit |
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| 别名 | B-raf 1; BRAF1; RAFB1; V-Raf Murine Sarcoma Viral Oncogene Homolog B1 |
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规格与价格
| 48T 价格 | 1820 |
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| 96T 价格 | 2600 |
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| 96T*5 价格 | 11050 |
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检测参数
| 种属 | Human |
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| 检测类型 | Sandwich |
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| 灵敏度 | 0.118 ng/mL |
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| 检测范围 | 0.32-20 ng/mL |
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| 样本类型 | tissue homogenates, cell lysates and other biological fluids |
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| 实验时长 | 3.5h |
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研究信息
| 研究领域 | Enzyme & Kinase; |
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| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BRAF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BRAF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BRAF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BRAF in the samples is then determined by comparing the OD of the samples to the standard curve. |
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| UniProt编号 | P15056 |
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标准曲线
| 标准曲线图 |  |
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