人弹性蛋白酶3B(ELA3B)微量上样酶联免疫吸附检测试剂盒

英文名称	Human ELA3B(Elastase 3B) Microsample ELISA Kit
别名	CELA3B; Chymotrypsin Like Elastase Family Member 3B; Protease E; Fecal Elastase; Elastase 1; Cholesterol-Binding Pancreatic Protease; Pancreatic Endopeptidase E

种属	Human
检测类型	Sandwich
灵敏度	0.129 ng/mL
检测范围	0.32-20 ng/mL
样本类型	Serum, plasma and other biological fluids.
实验时长	3.5h

研究领域	Enzyme & Kinase;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ELA3B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ELA3B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ELA3B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ELA3B in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	P08861

标准曲线图

【仅供科学研究使用】

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