小鼠β-位淀粉样前体蛋白裂解酶1(bACE1)酶联免疫吸附检测试剂盒

英文名称	Mouse bACE1(Beta-Site APP Cleaving Enzyme 1) ELISA Kit
别名	ASP2; Beta Secretase; Memapsin 2; Aspartyl protease 2; Beta-site amyloid precursor protein cleaving enzyme 1; Membrane-associated aspartic protease 2

种属	Mouse
检测类型	Sandwich
灵敏度	14.3 pg/mL
检测范围	31.25-2000 pg/mL
样本类型	Serum, plasma, tissue homogenates, cerebrospinal fluid and other biological fluids
实验时长	3.5h

研究领域	Neuro science;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse bACE1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse bACE1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse bACE1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse bACE1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	P56818

标准曲线图

【仅供科学研究使用】

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