小鼠次黄嘌呤磷酸核糖基转移酶1(HPRT1)酶联免疫吸附检测试剂盒

英文名称	Mouse HPRT1(Hypoxanthine Phosphoribosyltransferase 1) ELISA Kit
别名	HGPRT; HGPRTase; HPRT; Lesch-Nyhan Syndrome; Hypoxanthine-guanine phosphoribosyltransferase

种属	Mouse
检测类型	Sandwich
灵敏度	0.059 ng/mL
检测范围	0.16-10 ng/mL
样本类型	serum, plasma, tissue homogenates and other biological fluids
实验时长	3.5h

研究领域	Metabolic pathway;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse HPRT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse HPRT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse HPRT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse HPRT1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	P00493

标准曲线图

【仅供科学研究使用】

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