人磷酸甘油酸酯激酶1(PGK1)酶联免疫吸附检测试剂盒

英文名称	Human PGK1(Phosphoglycerate Kinase 1) ELISA Kit
别名	MIG10; PGKA; PRP 2; Cell migration-inducing gene 10 protein; Primer recognition protein 2

种属	Human
检测类型	Sandwich
灵敏度	12.4 pg/mL
检测范围	31.25-2000 pg/mL
样本类型	serum, plasma, tissue homogenates and other biological fluids
实验时长	3.5h

研究领域	Enzyme & Kinase;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PGK1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PGK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PGK1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PGK1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	P00558

标准曲线图

【仅供科学研究使用】

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