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大鼠性别决定区Y框蛋白2(SOX2)酶联免疫吸附检测试剂盒

  • 货号:ELK2978
  • 价格:¥2,600.00 元
  • 点击:1728

产品介绍

产品信息

英文名称Rat SOX2(Sex Determining Region Y Box Protein 2) ELISA Kit
别名ANOP3; MCOPS3; SRY-Box 2; Transcription factor SOX-2; SRY related HMG box gene 2; SRY related HMG box 2

规格与价格

48T 价格1820
96T 价格2600
96T*5 价格11050

检测参数

种属Rat
检测类型Sandwich
灵敏度0.063 ng/mL
检测范围0.16-10 ng/mL
样本类型Tissue homogenates, cell lysates and other biological fluids.
实验时长3.5h

研究信息

研究领域Reproductive science;Developmental science;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SOX2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SOX2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SOX2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SOX2 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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