小鼠碱性鞘磷脂磷酸二酯酶(Alk-Smase)酶联免疫吸附检测试剂盒

英文名称	Mouse Alk-Smase(Alkaline Sphingomyelinase) ELISA Kit
别名	ENPP7; E-NPP 7; NPP-7; Ectonucleotide Pyrophosphatase/Phosphodiesterase 7; Sphingomyelin Phosphodiesterase; Intestinal alkaline sphingomyelinase

种属	Mouse
检测类型	Sandwich
灵敏度	0.049 ng/mL
检测范围	0.16-10 ng/mL
样本类型	Tissue homogenates and other biological fluids.
实验时长	3.5h

研究领域	Enzyme & Kinase;Neuro science;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Alk-Smase. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Alk-Smase. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Alk-Smase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Alk-Smase in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	Q3TIW9

标准曲线图

【仅供科学研究使用】

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