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小鼠基质金属蛋白酶13(MMP13)酶联免疫吸附检测试剂盒

  • 货号:ELK2539
  • 价格:¥2,600.00 元
  • 点击:1576

产品介绍

产品信息

英文名称Mouse MMP13(Matrix Metalloproteinase 13) ELISA Kit
别名CLG3; Collagenase 3

规格与价格

48T 价格1820
96T 价格2600
96T*5 价格11050

检测参数

种属Mouse
检测类型Sandwich
灵敏度27 pg/mL
检测范围78.13-5000 pg/mL
样本类型serum, plasma and other biological fluids
实验时长3.5h

研究信息

研究领域Enzyme & Kinase;Tumor immunity;Bone metabolism;Rheumatology;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MMP13. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MMP13. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MMP13, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MMP13 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号P33435

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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