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小鼠内分泌腺来源血管内皮生长因子(EG-VEGF)酶联免疫吸附检测试剂盒

  • 货号:ELK2479
  • 价格:¥2,400.00 元
  • 点击:1931

产品介绍

产品信息

英文名称Mouse EG-VEGF(Endocrine Gland Derived Vascular Endothelial Growth Factor) ELISA Kit
别名PK1; EGVEGF; PRK1, PROK1; Prokineticin 1; Black Mamba Toxin-Related Protein; Mambakine

规格与价格

48T 价格1680
96T 价格2400
96T*5 价格10200

检测参数

种属Mouse
检测类型Sandwich
灵敏度5.8 pg/mL
检测范围15.63-1000 pg/mL
样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长3.5h

研究信息

研究领域Cytokine;Tumor immunity;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse EG-VEGF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse EG-VEGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse EG-VEGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse EG-VEGF in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号Q14A28

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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