大鼠内皮素1(ET-1)微量上样酶联免疫吸附检测试剂盒
- 货号：ELK2436MS
- 价格：￥2,600.00 元
- 点击：1301
产品介绍
产品信息
| 英文名称 | Rat ET-1(Endothelin1) Microsample ELISA Kit |
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| 别名 | ET1; PPET1; Preproendothelin-1; Big endothelin-1; EDN1 |
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规格与价格
| 48T 价格 | 1820 |
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| 96T 价格 | 2600 |
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| 96T*5 价格 | 11050 |
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检测参数
| 种属 | Rat |
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| 检测类型 | Sandwich |
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| 灵敏度 | 2.67 pg/mL |
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| 检测范围 | 7.82-500 pg/mL |
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| 样本类型 | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
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| 实验时长 | 3.5h |
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研究信息
| 研究领域 | Endocrinology;Cardiovascular biology; |
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| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ET-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ET-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ET-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ET-1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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| UniProt编号 | P22387 |
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标准曲线
| 标准曲线图 |  |
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