大鼠内皮素1(ET-1)酶联免疫吸附检测试剂盒

英文名称	Rat ET-1(Endothelin1) ELISA Kit
别名	ET1; PPET1; Preproendothelin-1; Big endothelin-1; EDN1

种属	Rat
检测类型	Sandwich
灵敏度	2.63 pg/mL
检测范围	7.82-500 pg/mL
样本类型	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长	3.5h

研究领域	Endocrinology;Cardiovascular biology;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ET-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ET-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ET-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ET-1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	P22387

标准曲线图

【仅供科学研究使用】

相关产品:

产品名称	点击数
牛17-羟孕酮(17-OHP)酶联免疫吸附检测试剂盒	1085
犬皮质醇(Cor)酶联免疫吸附检测试剂盒	1359
山羊降钙素原(PCT)酶联免疫吸附检测试剂盒	660
鸡胱天蛋白酶3(CASP3)酶联免疫吸附检测试剂盒	1621
鸡胱天蛋白酶8(CASP8)酶联免疫吸附检测试剂盒	854
犬分泌性白细胞蛋白酶抑制因子(SLPI)酶联免疫吸附检测试剂盒	1047
山羊整合素β1(ITGb1)酶联免疫吸附检测试剂盒	1429
猪三碘甲状腺原氨酸(T3)酶联免疫吸附检测试剂盒	996
猪甲状腺素(T4)酶联免疫吸附检测试剂盒	919
人I型前胶原(PCI)酶联免疫吸附检测试剂盒	857
猪雄烯二酮(ASD)酶联免疫吸附检测试剂盒	1115
人门冬酰胺酶(ASRGL1)酶联免疫吸附检测试剂盒	1162
山羊生长激素(GH)酶联免疫吸附检测试剂盒	1801
大鼠三碘甲状腺原氨酸(T3)酶联免疫吸附检测试剂盒	1042
大鼠甲状腺素(T4)酶联免疫吸附检测试剂盒	1575
人谷氨酸半胱氨酸连接酶催化亚基(GCLC)酶联免疫吸附检测试剂盒	1388
大鼠脂多糖(LPS)酶联免疫吸附检测试剂盒	1053
大鼠脂多糖(LPS)微量上样酶联免疫吸附检测试剂盒	1270
绵羊谷胱甘肽过氧化酶1(GPX1)酶联免疫吸附检测试剂盒	1228
绵羊谷胱甘肽过氧化酶2(GPX2)酶联免疫吸附检测试剂盒	1388