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人Ⅲ型胶原(COL3)微量上样酶联免疫吸附检测试剂盒

  • 货号:ELK2330MS
  • 价格:¥2,600.00 元
  • 点击:2047

产品介绍

产品信息

英文名称Human COL3(Collagen Type III) Microsample ELISA Kit
别名Collagen Type 3; Col 3; Collagen Type III; Col III

规格与价格

48T 价格1820
96T 价格2600
96T*5 价格11050

检测参数

种属Human
检测类型Sandwich
灵敏度0.235 ng/mL
检测范围0.63-40 ng/mL
样本类型serum, plasma, tissue homogenates and other biological fluids
实验时长3.5h

研究信息

研究领域Metabolic pathway;Cardiovascular biology;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human COL3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human COL3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human COL3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human COL3 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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