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人N-乙酰β-D-氨基葡萄糖苷酶(NAGase)酶联免疫吸附检测试剂盒

  • 货号:ELK1917
  • 价格:¥2,600.00 元
  • 点击:2283
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产品介绍

产品信息

英文名称Human NAGase(N-Acetyl Beta-D-Glucosaminidase) ELISA Kit
别名MGEA5; MEA5; NCOAT; Hexosaminidase C; N-Acetylhexosaminidase; Hyaluronidase; Meningioma-Expressed Antigen 5; Nuclear Cytoplasmic O-GlcNAcase And Acetyltransferase

规格与价格

48T 价格1820
96T 价格2600
96T*5 价格11050

检测参数

种属Human
检测类型Sandwich
灵敏度0.54 ng/mL
检测范围1.57-100 ng/mL
样本类型Urine
实验时长3.5h

研究信息

研究领域Enzyme & Kinase;Kidney biomarker;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NAGase. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NAGase. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NAGase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NAGase in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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