人凋亡蛋白酶激活因子1(APAF1)酶联免疫吸附检测试剂盒

英文名称	Human APAF1(Apoptotic Peptidase Activating Factor 1) ELISA Kit
别名	CED4; Apoptotic Protease Activating Factor

种属	Human
检测类型	Sandwich
灵敏度	0.058 ng/mL
检测范围	0.16-10 ng/mL
样本类型	Tissue homogenates, cell lysates and other biological fluids
实验时长	3.5h

研究领域	Apoptosis;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human APAF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human APAF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human APAF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human APAF1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	O14727

标准曲线图

【仅供科学研究使用】

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