| 英文名称 | Human AP12(Apelin 12) ELISA Kit |
|---|---|
| 别名 | APLN12 |
| 48T 价格 | 1820 |
|---|---|
| 96T 价格 | 2600 |
| 96T*5 价格 | 11050 |
| 种属 | Human |
|---|---|
| 检测类型 | Competitive Inhibition |
| 灵敏度 | 25.28 pg/mL |
| 检测范围 | 93.75-6000 pg/mL |
| 样本类型 | Serum, plasma, cell lysates, cell culture supernates and other biological fluids |
| 实验时长 | 2h |
| 研究领域 | Metabolic pathway;Endocrinology;Cardiovascular biology;Gastroenterology;Hormone metabolism; |
|---|---|
| 检测原理 | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human AP12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AP12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AP12 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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