小鼠诱导型一氧化氮合成酶(NOS2/iNOS)酶联免疫吸附检测试剂盒

英文名称	Mouse NOS2/iNOS(NitricOxide Synthase 2, Inducible) ELISA Kit
别名	NOS2A; INOS; HEP-NOS; I-NOS; Hepatocytes Oxide Synthase; Peptidyl-cysteine S-nitrosylase NOS2; NOS2; Nitric Oxide Synthase 2, Inducible

种属	Mouse
检测类型	Sandwich
灵敏度	0.116 ng/mL
检测范围	0.32-20 ng/mL
样本类型	tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长	3.5h

研究领域	Enzyme & Kinase;Metabolic pathway;Tumor immunity;Cardiovascular biology;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NOS2/iNOS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NOS2/iNOS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NOS2/iNOS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NOS2/iNOS in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	P29477

标准曲线图

【仅供科学研究使用】

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