人热休克蛋白40(HSP40)酶联免疫吸附检测试剂盒

英文名称	Human HSP40(Heat Shock Protein 40) ELISA Kit
别名	DnaJ; DNAJB1; Sis1; RSPH16B; HSPF1; DnaJ homolog subfamily B member 1; Radial Spoke 16 Homolog B; Heat Shock 40kDa Protein 1; DnaJ protein homolog 1

种属	Human
检测类型	Sandwich
灵敏度	0.055 ng/mL
检测范围	0.16-10 ng/mL
样本类型	tissue homogenates, cell lysates and other biological fluids
实验时长	3.5h

研究领域	Signal transduction;Developmental science;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HSP40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HSP40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HSP40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HSP40 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	P25685

标准曲线图

【仅供科学研究使用】

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